# Commission Regulation (EU) No 175/2010 of 2 March 2010 implementing Council Directive 2006/88/EC as regards measures to control increased mortality in oysters of the species Crassostrea gigas in connection with the detection of Ostreid herpesvirus 1 μvar (OsHV-1 μvar) (Text with EEA relevance), Division 4.2.3: PCR mix

Extent: E+W+S+N.I.

PCR mix for each tube is:
Volume per tube
Final concentration
Buffer (10 X)
5 μl
1 X
MgCl2 (25 mM)
5 μl
2,5 mM
dNTP (2 mM)
5 μl
0,2 mM
CF (10 μM)
1 μl
0,2 μM
CR (10 μM)
1 μl
0,2 μM
Taq polymérase (5U/μl)
0,5 μl
2,5 U
dH2O
31,5 μl
49 μl of this PCR mix is dispensed in each PCR tube
1 μl of extracted DNA (50-100 ng/μl) is added to each tube

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Source: https://www.legislation.gov.uk/eur/2010/175/annex/I/part/B/division/4/division/4.2/division/4.2.3 (the official version)
Page: https://openlegislation.uk/eu-regulation-2010-175/annex-I/part-B/division-4/division-4.2/division-4.2.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.