{
  "url": "https://openlegislation.uk/eu-regulation-2010-175/annex-I/part-B/division-4/division-4.2/division-4.2.4",
  "json_url": "https://openlegislation.uk/eu-regulation-2010-175/annex-I/part-B/division-4/division-4.2/division-4.2.4.json",
  "legislation": "eur/2010/175",
  "legislation_title": "Commission Regulation (EU) No 175/2010 of 2 March 2010 implementing Council Directive 2006/88/EC as regards measures to control increased mortality in oysters of the species Crassostrea gigas in connection with the detection of Ostreid herpesvirus 1 μvar (OsHV-1 μvar) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2010-175",
  "path": "annex/I/part/B/division/4/division/4.2/division/4.2.4",
  "kind": "division",
  "number": "4.2.4",
  "heading": "Controls",
  "text": "Two types of control are used:\nNegative controls consist of dH2O (1 μl for 49 μl of PCR mix). They aim at detecting potential reactive contamination or working environment. One negative control should be included every 10 samples or after each batch of samples.\nPositive controls consist of plasmidic DNA containing the OsHV-1 target genome region CF-CR. They aim at checking the efficacy of the PCR reaction. One positive control should be included for each PCR analysis. Positive controls are available from the Community Reference Laboratory.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2010/175/annex/I/part/B/division/4/division/4.2/division/4.2.4",
    "retrieved_at": "2026-11-01T14:50:54.654314+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}