{
  "url": "https://openlegislation.uk/eu-regulation-2010-175/annex-I/part-B/division-4/division-4.2/division-4.2.5",
  "json_url": "https://openlegislation.uk/eu-regulation-2010-175/annex-I/part-B/division-4/division-4.2/division-4.2.5.json",
  "legislation": "eur/2010/175",
  "legislation_title": "Commission Regulation (EU) No 175/2010 of 2 March 2010 implementing Council Directive 2006/88/EC as regards measures to control increased mortality in oysters of the species Crassostrea gigas in connection with the detection of Ostreid herpesvirus 1 μvar (OsHV-1 μvar) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2010-175",
  "path": "annex/I/part/B/division/4/division/4.2/division/4.2.5",
  "kind": "division",
  "number": "4.2.5",
  "heading": "Amplification",
  "text": "Amplification cycles are performed in a thermal cycle apparatus.\nInitial denaturation: 2 min at 94 °C\nAmplification: 35 cycles (1 min at 94 °C, 1 min at 50 °C and 1 min at 72 °C)\nFinal elongation: 5 min at 72 °C",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2010/175/annex/I/part/B/division/4/division/4.2/division/4.2.5",
    "retrieved_at": "2026-11-01T14:50:54.654314+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}