# Commission Regulation (EU) No 175/2010 of 2 March 2010 implementing Council Directive 2006/88/EC as regards measures to control increased mortality in oysters of the species Crassostrea gigas in connection with the detection of Ostreid herpesvirus 1 μvar (OsHV-1 μvar) (Text with EEA relevance), Division 4.3.1: Reactives

Extent: E+W+S+N.I.

50 X TAE (can be bought directly ready for use):
Tris base (40 mM) 242 g
Acetic glacial acid (40 mM) 57,1 ml
Na2EDTA.2H2O (1 mM) 18,61 g
dH2O for 1 liter
Ajust at pH 8
Agarose gel 2,5 % in 1 X TAE
Ethidium bromide (0,5 μg/ml) added after cooling the gel.
Loading blue dye:
Bromophenol blue 0,25 %
Cyanol xylene FF 0,25 %
Sucrose 40 %
Keep at 4 °C.
Use diluted 6 times (2 μl of loading blue buffer for 10μl of PCR products).
Molecular weight marker:
SmartLadder SF (Eurogentec): a ready-to-use molecular weight marker including 9 regularly spaced bands from 100 to 1 000 bp.

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Source: https://www.legislation.gov.uk/eur/2010/175/annex/I/part/B/division/4/division/4.3/division/4.3.1 (the official version)
Page: https://openlegislation.uk/eu-regulation-2010-175/annex-I/part-B/division-4/division-4.3/division-4.3.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.