{
  "url": "https://openlegislation.uk/eu-regulation-2010-175/annex-I/part-B/division-4/division-4.3/division-4.3.2",
  "json_url": "https://openlegislation.uk/eu-regulation-2010-175/annex-I/part-B/division-4/division-4.3/division-4.3.2.json",
  "legislation": "eur/2010/175",
  "legislation_title": "Commission Regulation (EU) No 175/2010 of 2 March 2010 implementing Council Directive 2006/88/EC as regards measures to control increased mortality in oysters of the species Crassostrea gigas in connection with the detection of Ostreid herpesvirus 1 μvar (OsHV-1 μvar) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2010-175",
  "path": "annex/I/part/B/division/4/division/4.3/division/4.3.2",
  "kind": "division",
  "number": "4.3.2",
  "heading": "Agarose gel preparation",
  "text": "1.. Weight 2,5 g of agarose, add 100 ml of 1 X TAE and heat until the mix is melted.\n2.. After cooling the solution, ethidium bromide is added (5 μl for 100 ml of agarose gel) and the solution is disposed in a specific mould equipped with combs (to form slots).\n3.. When gel is polymerised, combs are removed and gel is placed in a horizontal electrophoresis system containing enough 1 X TAE to the cover agarose gel.\n4.. 10 μl of PCR products are mixed with 2 μl of blue dye (6 X) and disposed in the slots.\n5.. One whole is dedicated to the molecular weight marker (5 μl).\n6.. A voltage of 50 to 150 volts is applied during 30 min to 1 hour depending on the gel size and thickness.\n7.. Gel is observed under UV.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2010/175/annex/I/part/B/division/4/division/4.3/division/4.3.2",
    "retrieved_at": "2026-11-01T14:50:54.654314+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}