# Commission Regulation (EU) No 175/2010 of 2 March 2010 implementing Council Directive 2006/88/EC as regards measures to control increased mortality in oysters of the species Crassostrea gigas in connection with the detection of Ostreid herpesvirus 1 μvar (OsHV-1 μvar) (Text with EEA relevance), Division 4.3.2: Agarose gel preparation

Extent: E+W+S+N.I.

1.. Weight 2,5 g of agarose, add 100 ml of 1 X TAE and heat until the mix is melted.
2.. After cooling the solution, ethidium bromide is added (5 μl for 100 ml of agarose gel) and the solution is disposed in a specific mould equipped with combs (to form slots).
3.. When gel is polymerised, combs are removed and gel is placed in a horizontal electrophoresis system containing enough 1 X TAE to the cover agarose gel.
4.. 10 μl of PCR products are mixed with 2 μl of blue dye (6 X) and disposed in the slots.
5.. One whole is dedicated to the molecular weight marker (5 μl).
6.. A voltage of 50 to 150 volts is applied during 30 min to 1 hour depending on the gel size and thickness.
7.. Gel is observed under UV.

---
Source: https://www.legislation.gov.uk/eur/2010/175/annex/I/part/B/division/4/division/4.3/division/4.3.2 (the official version)
Page: https://openlegislation.uk/eu-regulation-2010-175/annex-I/part-B/division-4/division-4.3/division-4.3.2
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.