# Commission Regulation (EU) No 200/2010 of 10 March 2010 implementing Regulation (EC) No 2160/2003 of the European Parliament and of the Council as regards a Union target for the reduction of the prevalence of Salmonella serotypes in adult breeding flocks of Gallus gallus (Text with EEA relevance), Division 3.1.3: Boot swabs and dust samples:

Extent: E+W+S+N.I.

(a)The pair(s) of boot/sock swabs and dust sample (fabric swab) shall be carefully unpacked to avoid dislodging adherent faecal material or loose dust material and placed in 225 ml of BPW which has been pre-warmed to room temperature.
(b)The boot/socks and fabric swab shall be fully submersed in BPW to provide sufficient free liquid around the sample for migration of Salmonella away from the sample and therefore more BPW may be added, if necessary.
Separate preparations must be made of the boot swabs and the fabric swab.
(c)Where five pairs of boot/sock swabs are pooled into two samples, each pooled sample must be placed in of 225 ml of BPW, or more if necessary, to fully submerge the sample and provide sufficient free liquid around the sample for migration of Salmonella away from the sample.
(d)Swirl to fully saturate the sample and continue the culture by using the detection method described in point 3.2.

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Source: https://www.legislation.gov.uk/eur/2010/200/annex/division/3/division/3.1/division/3.1.3 (the official version)
Page: https://openlegislation.uk/eu-regulation-2010-200/annex/division-3/division-3.1/division-3.1.3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.