# Commission Regulation (EU) No 200/2010 of 10 March 2010 implementing Regulation (EC) No 2160/2003 of the European Parliament and of the Council as regards a Union target for the reduction of the prevalence of Salmonella serotypes in adult breeding flocks of Gallus gallus (Text with EEA relevance), Division 3.1.4: Other faecal material samples:

Extent: E+W+S+N.I.

(a)The faeces samples shall be pooled and thoroughly mixed and a 25 g sub-sample shall be collected for culture.
(b)The 25 g sub-sample shall be added to 225 ml of BPW which has been pre-warmed to room temperature.
(c)The culture of the sample shall be continued by using the detection method described in point 3.2.
If ISO standards on the preparation of relevant samples for the detection of Salmonella are agreed on, they shall be applied and replace those referred to in points 3.1.2, 3.1.3 and 3.1.4 on sampling preparation.

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Source: https://www.legislation.gov.uk/eur/2010/200/annex/division/3/division/3.1/division/3.1.4 (the official version)
Page: https://openlegislation.uk/eu-regulation-2010-200/annex/division-3/division-3.1/division-3.1.4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.