{
  "url": "https://openlegislation.uk/eu-regulation-2010-200/annex/division-3/division-3.2",
  "json_url": "https://openlegislation.uk/eu-regulation-2010-200/annex/division-3/division-3.2.json",
  "legislation": "eur/2010/200",
  "legislation_title": "Commission Regulation (EU) No 200/2010 of 10 March 2010 implementing Regulation (EC) No 2160/2003 of the European Parliament and of the Council as regards a Union target for the reduction of the prevalence of Salmonella serotypes in adult breeding flocks of Gallus gallus (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2010-200",
  "path": "annex/division/3/division/3.2",
  "kind": "division",
  "number": "3.2",
  "heading": "Detection method",
  "text": "The detection of the relevant Salmonella serotypes must be carried out according to ISO 6579-1 as it has effect for the time being.\nAs regards the boot swabs samples, dust samples and other faecal material samples referred to in point 3.1, the incubated BPW enrichment broth for future culture may be pooled. To do so, incubate both samples in BPW as referred to in point 3.1.3. Take 1 ml of incubated broth from each sample and mix thoroughly, then take 0,1 ml of the mixture and inoculate the modified semi-solid Rappaport-Vassiliadis (MSRV) plates.\nThe samples in BPW must not be shaken, swirled or otherwise agitated after incubation as this releases inhibitory particulates and reduces subsequent isolation in MSRV.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Words in Annex point 3.2 substituted (31.12.2020) by The Zoonotic Disease Eradication and Control (Amendment) (EU Exit) Regulations 2019 (S.I. 2019/740), regs. 1, 7(11); 2020 c. 1, Sch. 5 para. 1(1)"
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2010/200/annex/division/3/division/3.2",
    "retrieved_at": "2026-11-01T14:50:53.850237+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}