{
  "url": "https://openlegislation.uk/eu-regulation-2010-515/annex",
  "json_url": "https://openlegislation.uk/eu-regulation-2010-515/annex.json",
  "legislation": "eur/2010/515",
  "legislation_title": "Commission Regulation (EU) No 515/2010 of 15 June 2010 amending Regulation (EC) No 1137/2007 as regards the use of the feed additive Bacillus subtilis (O35) in feed containing lasalocid sodium, maduramycin ammonium, monensin sodium, narasin, salinomycin sodium and semduramycin sodium (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2010-515",
  "path": "annex",
  "kind": "annex",
  "number": null,
  "heading": null,
  "text": "‘ANNEX\nDetails of the analytical methods are available at the following address of the Community Reference Laboratory: www.irmm.jrc.be/crl-feed-additives’.\nIdentification number of the additive\nName of the holder of authorisation\nAdditive\nComposition, chemical formula, description, analytical method\nSpecies or category of animal\nMaximum age\nMinimum content\nMaximum content\nOther provisions\nEnd of period of authorisation\nCFU/kg of complete feedingstuff with a moisture content of 12 %\nCategory of zootechnical additives. Functional group: gut flora stabilisers\n4b1821\nChr. Hansen A/S\nBacillus subtilis DSM 17299\nAdditive composition\nPreparation of Bacillus subtilis DSM 17299 containing a minimum of 1,6 × 109 CFU/g of additive\nCharacterisation of the active sustance\nBacillus subtilis DSM 17299 spore concentrate\nAnalytical method\nEnumeration spread plate method using tryptone soya agar with preheat treatment of feed samples\nChickens for fattening\n—\n8 × 108\n1,6 × 109\n1.\nIn the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.\n2.\nThe use is permitted in feed containing the permitted coccidiostats: diclazuril, halofuginone, robenidine, decoquinate, narasin/nicarbazin, lasalocid sodium, maduramycin ammonium, monensin sodium, narasin, salinomycin sodium or semduramycin sodium.\n22.10.2017",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "footnote",
      "label": "f1",
      "text": "Details of the analytical methods are available at the following address of the Community Reference Laboratory: www.irmm.jrc.be/crl-feed-additives’."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2010/515/annex",
    "retrieved_at": "2026-11-01T14:50:45.515591+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}