# Commission Regulation (EU) No 515/2010 of 15 June 2010 amending Regulation (EC) No 1137/2007 as regards the use of the feed additive Bacillus subtilis (O35) in feed containing lasalocid sodium, maduramycin ammonium, monensin sodium, narasin, salinomycin sodium and semduramycin sodium (Text with EEA relevance), Annex

Extent: E+W+S+N.I.

‘ANNEX
Details of the analytical methods are available at the following address of the Community Reference Laboratory: www.irmm.jrc.be/crl-feed-additives’.
Identification number of the additive
Name of the holder of authorisation
Additive
Composition, chemical formula, description, analytical method
Species or category of animal
Maximum age
Minimum content
Maximum content
Other provisions
End of period of authorisation
CFU/kg of complete feedingstuff with a moisture content of 12 %
Category of zootechnical additives. Functional group: gut flora stabilisers
4b1821
Chr. Hansen A/S
Bacillus subtilis DSM 17299
Additive composition
Preparation of Bacillus subtilis DSM 17299 containing a minimum of 1,6 × 109 CFU/g of additive
Characterisation of the active sustance
Bacillus subtilis DSM 17299 spore concentrate
Analytical method
Enumeration spread plate method using tryptone soya agar with preheat treatment of feed samples
Chickens for fattening
—
8 × 108
1,6 × 109
1.
In the directions for use of the additive and premixture, indicate the storage temperature, storage life and stability to pelleting.
2.
The use is permitted in feed containing the permitted coccidiostats: diclazuril, halofuginone, robenidine, decoquinate, narasin/nicarbazin, lasalocid sodium, maduramycin ammonium, monensin sodium, narasin, salinomycin sodium or semduramycin sodium.
22.10.2017

---
Source: https://www.legislation.gov.uk/eur/2010/515/annex (the official version)
Page: https://openlegislation.uk/eu-regulation-2010-515/annex
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.