{
  "url": "https://openlegislation.uk/eu-regulation-2012-200/annex/division-3/division-3.1",
  "json_url": "https://openlegislation.uk/eu-regulation-2012-200/annex/division-3/division-3.1.json",
  "legislation": "eur/2012/200",
  "legislation_title": "Commission Regulation (EU) No 200/2012 of 8 March 2012 concerning a Union target for the reduction of Salmonella enteritidis and Salmonella typhimurium in flocks of broilers, as provided for in Regulation (EC) No 2160/2003 of the European Parliament and of the Council (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2012-200",
  "path": "annex/division/3/division/3.1",
  "kind": "division",
  "number": "3.1",
  "heading": "Preparation of the samples",
  "text": "At the laboratory samples shall be kept refrigerated until examination. Examination shall start within 48 hours following the time of receipt of the samples and within four days from the date of sampling.\nDust samples shall be analysed separately. However, the competent authority may decide to pool them with the pair of boot swabs for analysis.\nThe sample shall be swirled to fully saturate it and culturing shall be continued by using the detection method set out in point 3.2.\nThe two pairs of boot swabs shall be carefully unpacked to avoid dislodging adherent faecal material, pooled and placed in 225 ml of buffered peptone water (BPW) pre-warmed to room temperature, or 225 ml of diluent is added directly to the two pairs of boot swabs in their container as received in the laboratory.\nThe boot swabs shall be fully submersed in BPW to provide sufficient free liquid around the sample for migration of Salmonella away from the sample and therefore more BPW may be added if necessary.\nIf ISO standards on the preparation of faeces for the detection of Salmonella are agreed on, they shall replace the provisions on the preparation of samples set out in this point as appropriate.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Word in Annex point 3.1 substituted (31.12.2020) by The Zoonotic Disease Eradication and Control (Amendment) (EU Exit) Regulations 2019 (S.I. 2019/740), regs. 1, 9(5)(e); 2020 c. 1, Sch. 5 para. 1(1)"
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2012/200/annex/division/3/division/3.1",
    "retrieved_at": "2026-11-01T14:49:32.612660+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}