{
  "url": "https://openlegislation.uk/eu-regulation-2013-739/annex-II",
  "json_url": "https://openlegislation.uk/eu-regulation-2013-739/annex-II.json",
  "legislation": "eur/2013/739",
  "legislation_title": "Commission Regulation (EU) No 739/2013 of 30 July 2013 amending Annex II to Regulation (EC) No 1333/2008 of the European Parliament and of the Council as regards the use of Stigmasterol-rich plant sterols as a stabiliser in ready-to-freeze alcoholic cocktails, and the Annex to Commission Regulation (EU) No 231/2012 as regards specifications for Stigmasterol-rich plant sterols food additive (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2013-739",
  "path": "annex/II",
  "kind": "annex",
  "number": "II",
  "heading": null,
  "text": "In the Annex to Regulation (EU) No 231/2012 the following entry for E 499 is inserted after the entry for E 495:\n‘\nE 499 STIGMASTEROL-RICH PLANT STEROLS\nSynonyms\nDefinition\nStigmasterol-rich plant sterols are derived from soybeans and are a chemically defined simple mixture that comprises not less than 95 % of plant sterols (stigmasterol, β-sitosterol, campesterol and brassicasterol), with stigmasterol representing not less than 85 % of the stigmasterol-rich plant sterols.\nEinecs\nChemical name\nStigmasterol\n(3S,8S,9S,10R,13R,14S,17R)-17-(5-ethyl-6-methyl-hept-3-en-2-yl)-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1Hcyclopenta[a]phenanthren-3-ol\nβ-Sitosterol\n(3S,8S,9S,10R,13R,14S,17R)-17-[(2S,5S)-5-ethyl-6-methylheptan-2-yl]-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1Hcyclopenta[a]phenanthren-3-ol\nCampesterol\n(3S,8S,9S,10R,13R,14S,17R)-17-(5,6-dimethylheptan-2-yl)-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1Hcyclopenta[a]phenanthren-3-ol\nBrassicasterol\n(3S,8S,9S,10R,13R,14S,17R)-17-[(E,2R,5R)-5,6-dimethylhept-3-en-2-yl]-10,13-dimethyl-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1Hcyclopenta[a]phenanthren-3-ol\nChemical formula\nStigmasterol\nC29H48O\nβ-Sitosterol\nC29H50O\nCampesterol\nC28H48O\nBrassicasterol\nC28H46O\nMolecular weight\nStigmasterol\n412,6 g/mol\nβ-Sitosterol\n414,7 g/mol\nCampesterol\n400,6 g/mol\nBrassicasterol\n398,6 g/mol\nAssay (products containing only free sterols and stanols)\nContent not less than 95 % on a total free sterol/stanol basis on the anhydrous basis\nDescription\nFree-flowing, white to off-white powders, pills or pastilles; colourless to pale yellow liquids\nIdentification\nSolubility\nPractically insoluble in water. Phytosterols and phytostanols are soluble in acetone and ethyl acetate.\nStigmasterol content\nNot less than 85 % (w/w)\nOther plant sterols/stanols: either singularly or in combination including Brassicasterol, campestanol, campesterol, Δ-7-campesterol, cholesterol, chlerosterol, sitostanol and β-sitosterol.\nNot more than 15 % (w/w)\nPurity\nTotal Ash\nNot more than 0,1 %\nResidual Solvents\nEthanol: Not more than 5 000 mg/kg\nMethanol: Not more than 50 mg/kg\nWater content\nNot more than 4 % (Karl Fischer method)\nArsenic\nNot more than 3 mg/kg\nLead\nNot more than 1 mg/kg\nMicrobiological criteria\nTotal plate count\nNot more than 1 000 CFU/g\nYeasts\nNot more than 100 CFU/g\nMoulds\nNot more than 100 CFU/g\nEscherichia coli\nNot more than 10 CFU/g\nSalmonella spp.\nAbsent in 25 g\n’",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2013/739/annex/II",
    "retrieved_at": "2026-11-01T14:48:29.911047+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}