Annex II

United Kingdom

In the Annex to Regulation (EU) No 231/2012, the following entry is inserted after the specifications for food additive E 422:

‘ E 423 OCTENYL SUCCINIC ACID MODIFIED GUM ARABIC

Synonyms Gum arabic hydrogen octenylbutandioate; Gum arabic hydrogen octenylsuccinate; OSA modified gum arabic; OSA modified gum acacia
Definition Octenyl succinic acid modified gum arabic is produced by esterifying gum arabic (Acacia seyal), or gum arabic (Acacia senegal) in aqueous solution with not more than 3 % of octenyl succinic acid anhydride. It is subsequently spray dried.
Einecs
Chemical name
Chemical formula
Weight Average Molecular Weight
Fraction (i): 3,105 g/mol

Fraction (ii) 1,106 g/mol

Assay
Description Off-white to light tan, free flowing powder
Identification
Viscosity of a 5 % solution at 25 °C Not more than 30 mPa.s.
Precipitation reaction Forms flocculent precipitate in lead sub-acetate solution (TS)
Solubility Freely soluble in water; insoluble in ethanol
pH for a 5 % aqueous solution 3,5 to 6,5
Purity
Loss on drying Not more than 15 % (105 °C, 5 h)
Degree of esterification Not more than 0,6 %
Total ash Not more than 10 % (530 °C)
Acid-insoluble ash Not more than 0,5 %
Water insoluble matter Not more than 1,0 %
Test for starch or dextrine Boil a 1 in 50 aqueous solution of the sample, add about 0,1 ml iodine TS. No bluish or reddish colour should be produced.
Test for tannin-bearing gums To 10 ml of a 1 in 50 aqueous solution of the sample add about 0,1 ml ferric chloride TS. No blackish coloration or blackish precipitate should be formed.
Residual octenyl succinic acid Not more than 0,3 %
Lead Not more than 2 mg/kg
Microbiological criteria
Salmonella sp. Absent in 25 g
Escherichia coli Absent in 1 g

’

Identification
Synonyms	Gum arabic hydrogen octenylbutandioate; Gum arabic hydrogen octenylsuccinate; OSA modified gum arabic; OSA modified gum acacia
Definition	Octenyl succinic acid modified gum arabic is produced by esterifying gum arabic (Acacia seyal), or gum arabic (Acacia senegal) in aqueous solution with not more than 3 % of octenyl succinic acid anhydride. It is subsequently spray dried.
Einecs
Chemical name
Chemical formula
Weight Average Molecular Weight	Fraction (i): 3,105 g/mol Fraction (ii) 1,106 g/mol
Assay
Description	Off-white to light tan, free flowing powder
Viscosity of a 5 % solution at 25 °C	Not more than 30 mPa.s.
Precipitation reaction	Forms flocculent precipitate in lead sub-acetate solution (TS)
Solubility	Freely soluble in water; insoluble in ethanol
pH for a 5 % aqueous solution	3,5 to 6,5
Purity
Loss on drying	Not more than 15 % (105 °C, 5 h)
Degree of esterification	Not more than 0,6 %
Total ash	Not more than 10 % (530 °C)
Acid-insoluble ash	Not more than 0,5 %
Water insoluble matter	Not more than 1,0 %
Test for starch or dextrine	Boil a 1 in 50 aqueous solution of the sample, add about 0,1 ml iodine TS. No bluish or reddish colour should be produced.
Test for tannin-bearing gums	To 10 ml of a 1 in 50 aqueous solution of the sample add about 0,1 ml ferric chloride TS. No blackish coloration or blackish precipitate should be formed.
Residual octenyl succinic acid	Not more than 0,3 %
Lead	Not more than 2 mg/kg
Microbiological criteria
Salmonella sp.	Absent in 25 g
Escherichia coli	Absent in 1 g