# Commission Implementing Regulation (EU) 2015/1375 of 10 August 2015 laying down specific rules on official controls for Trichinella in meat (Codification) (Text with EEA relevance), Division I: Digestion procedure

Extent: E+W+S+N.I.

(a)Place the blender with the filtration insert, connect the waste tube and place the tube so it drains into the waste bin.
(b)When the blender is switched on, heating will start.
(c)Before this is done, the bottom valve located below the reaction chamber must be opened and closed.
(d)Up to 35 samples weighing approximately 1 g each (at 25 to 30 °C) taken from each individual sample in accordance with point 2 are then added. Ensure that larger pieces of tendons are removed as they may clot the membrane filter.
(e)Pour water up to the edge of a liquid chamber connected to the blender (approximately 400 ml).
(f)Pour about 30 ml hydrochloric acid (8,5 %) to the edge of the smaller, connected liquid chamber.
(g)Place a membrane filter under the coarse filter in the filter holder in the filter insert.
(h)Lastly, add 7 g of pepsin or 21 ml liquid pepsin. This order must be followed strictly to avoid decomposition of the pepsin.
(i)Close the lids of the reaction and liquid chambers.
(j)Select the period of digestion. A short digestion period (5 minutes) must be set for pigs at the normal slaughter age and a longer time (8 minutes) for other samples.
(k)When the start button on the blender is turned on, the process of dispensing and digestion starts automatically, followed by filtration. After 10 to 13 minutes the process is completed and stops automatically.
(l)Open the lid of the reaction chamber after checking that the chamber is empty. If there is foam or any digestion liquid remaining in the chamber, repeat the procedure in accordance with Section V.

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Source: https://www.legislation.gov.uk/eur/2015/1375/annex/I/chapter/II/division/C/division/3/division/I (the official version)
Page: https://openlegislation.uk/eu-regulation-2015-1375/annex-I/chapter-II/division-C/division-3/division-I
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.