{
  "url": "https://openlegislation.uk/eu-regulation-2015-1375/annex-I/chapter-II/division-D/division-1",
  "json_url": "https://openlegislation.uk/eu-regulation-2015-1375/annex-I/chapter-II/division-D/division-1.json",
  "legislation": "eur/2015/1375",
  "legislation_title": "Commission Implementing Regulation (EU) 2015/1375 of 10 August 2015 laying down specific rules on official controls for Trichinella in meat (Codification) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2015-1375",
  "path": "annex/I/chapter/II/division/D/division/1",
  "kind": "division",
  "number": "1",
  "heading": "Apparatus and reagents",
  "text": "(a)Knife or scissors and tweezers for cutting specimens.\n(b)Trays marked off into 50 squares, each of which can hold samples of approximately 2 g of meat, or other tools giving equivalent guarantees as regards the traceability of the samples.\n(c)A blender with a sharp chopping blade. Where the samples are larger than 3 g, a meat mincer with openings of 2 to 4 mm or scissors must be used. In the case of frozen meat or tongue (after removal of the superficial layer, which cannot be digested), a meat mincer is necessary and the sample size will need to be increased considerably.\n(d)Magnetic stirrers with thermostatically controlled heating plate and Teflon-coated stirring rods approximately 5 cm long.\n(e)Glass beakers, capacity 3 litres.\n(f)Sieves, mesh size 180 microns, external diameter 11 cm, with stainless steel mesh.\n(g)Steel filtration apparatus for 20 μm mesh filters with a steel funnel.\n(h)Vacuum pump.\n(i)Metal or plastic tanks, capacity 10 to 15 litres, to collect the digestive juice.\n(j)A 3D gyratory rocker.\n(k)Aluminium foil.\n(l)25 % hydrochloric acid.\n(m)Pepsin, strength: 1:10 000 NF (US National Formulary) corresponding to 1:12 500 BP (British Pharmacopoeia) and to 2 000 FIP (Fédération internationale de pharmacie), or stabilised liquid pepsin with minimum 660 European Pharmacopoeia units/ml.\n(n)Tap water heated to 46 to 48 °C.\n(o)A balance accurate to 0,1 g.\n(p)Pipettes of different sizes (1, 10 and 25 ml), micropipettes according to the latex agglutination manufacturer's instructions and pipette holders.\n(q)20 microns nylon mesh filters of a diameter that fits with the filtration system.\n(r)Plastic or steel forceps of 10 to 15 cm.\n(s)Conical vials of 15 ml.\n(t)A pestle with a Teflon or steel conical tip to fit in the conical vials.\n(u)A thermometer accurate to 0,5 °C within the range 1 to 100 °C.\n(v)Latex agglutination cards of the Trichin-L antigen test kit validated under the code No EURLP_D_001/2011.\n(w)Buffer solution with preservative (sample diluent) of the Trichin-L antigen test kit validated under the code No EURLP_D_001/2011.\n(x)Buffer supplemented with preservative (negative control) of the Trichin-L antigen test kit validated under the code No EURLP_D_001/2011.\n(y)Buffer supplemented with Trichinella spiralis antigens and preservative (positive control) of the Trichin-L antigen test kit validated under the code No EURLP_D_001/2011.\n(z)Buffer with polystyrene particles coated with antibodies supplemented with preservative (latex beads) of the Trichin-L antigen test kit validated under the code No EURLP_D_001/2011.\n(aa)Disposable sticks.",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2015/1375/annex/I/chapter/II/division/D/division/1",
    "retrieved_at": "2026-11-01T14:46:31.881114+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}