{
  "url": "https://openlegislation.uk/eu-regulation-2016-1240/division-1/division-6./division-6.4./division-6.4.3.",
  "json_url": "https://openlegislation.uk/eu-regulation-2016-1240/division-1/division-6./division-6.4./division-6.4.3..json",
  "legislation": "eur/2016/1240",
  "legislation_title": "Commission Implementing Regulation (EU) 2016/1240 of 18 May 2016 laying down rules for the application of Regulation (EU) No 1308/2013 of the European Parliament and of the Council with regard to public intervention and aid for private storage (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2016-1240",
  "path": "division/1/division/6./division/6.4./division/6.4.3.",
  "kind": "division",
  "number": "6.4.3",
  "heading": "Destaining the gel plate",
  "text": "Pour off the staining solution, wash the gel plate twice using a 100 ml destaining solution (4.10) each time, then shake with 200 ml destaining solution for 15 minutes and repeat the destaining step at least two or three times until the background is clear and uncoloured. Then rinse the gel plate with distilled water (2 × 2 minutes) and dry in the air (2 to 3 hours) or with a hairdryer (10 to 15 minutes).",
  "extent": null,
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "F",
      "label": "F1",
      "text": "Inserted by Commission Implementing Regulation (EU) 2018/150 of 30 January 2018 amending Implementing Regulation (EU) 2016/1240 as regards methods for the analysis and quality evaluation of milk and milk products eligible for public intervention and aid for private storage."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2016/1240/division/1/division/6./division/6.4./division/6.4.3.",
    "retrieved_at": "2026-11-01T14:45:39.577408+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}