{
  "url": "https://openlegislation.uk/eu-regulation-2016-1814/annex",
  "json_url": "https://openlegislation.uk/eu-regulation-2016-1814/annex.json",
  "legislation": "eur/2016/1814",
  "legislation_title": "Commission Regulation (EU) 2016/1814 of 13 October 2016 amending the Annex to Regulation (EU) No 231/2012 laying down specifications for food additives listed in Annexes II and III to Regulation (EC) No 1333/2008 of the European Parliament and of the Council as regards specifications for steviol glycosides (E 960) (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2016-1814",
  "path": "annex",
  "kind": "annex",
  "number": null,
  "heading": null,
  "text": "In the Annex to Regulation (EU) No 231/2012, the entry for E 960 steviol glycosides is replaced by the following:\n‘\nE 960 STEVIOL GLYCOSIDES\nSynonyms\nDefinition\nThe manufacturing process comprises two main phases: the first involving water extraction of the leaves of the Stevia rebaudiana Bertoni plant and preliminary purification of the extract by employing ion exchange chromatography to yield a steviol glycoside primary extract, and the second involving recrystallisation of the steviol glycosides from methanol or aqueous ethanol resulting in a final product containing not less than 95 % of the below identified 11 related steviol glycosides, in any combination and ratio.\nThe additive may contain residues of ion-exchange resins used in the manufacturing process. Several other related steviol glycosides that may be generated as a result of the production process, but do not occur naturally in the Stevia rebaudiana plant have been identified in small amounts (0,10 to 0,37 % w/w).\nChemical name\nSteviolbioside: 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid\nRubusoside: 13-β-D-glucopyranosyloxykaur-16-en-18-oic acid, β-D-glucopyranosyl ester\nDulcoside A: 13-[(2-O-α–L-rhamnopyranosyl-β–D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D-glucopyranosyl ester\nStevioside: 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D-glucopyranosyl ester\nRebaudioside A: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D- glucopyranosyl ester\nRebaudioside B: 13-[(2-O-β–D-glucopyranosyl-3-O-β–D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid\nRebaudioside C: 13-[(2-O-α–L-rhamnopyranosyl-3-O-β–D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D-glucopyranosyl ester\nRebaudioside D: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester\nRebaudioside E: 13-[(2-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester\nRebaudioside F: 13[(2-O-β-D-xylofurananosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, β-D-glucopyranosyl ester\nRebaudioside M: 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy]kaur-16-en-18-oic acid, 2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl ester\nMolecular formula\nTrivial name\nFormula\nConversion factor\nSteviol\nC20 H30 O3\n1,00\nSteviolbioside\nC32 H50 O13\n0,50\nRubusoside\nC32 H50 O13\n0,50\nDulcoside A\nC38 H60 O17\n0,40\nStevioside\nC38 H60 O18\n0,40\nRebaudioside A\nC44 H70 O23\n0,33\nRebaudioside B\nC38 H60 O18\n0,40\nRebaudioside C\nC44 H70 O22\n0,34\nRebaudioside D\nC50 H80 O28\n0,29\nRebaudioside E\nC44 H70 O23\n0,33\nRebaudioside F\nC43 H68 O22\n0,34\nRebaudioside M\nC56 H90 O33\n0,25\nMolecular weight and CAS No\nTrivial name\nCAS Number\nMolecular weight (g/mol)\nSteviol\n318,46\nSteviolbioside\n41093-60-1\n642,73\nRubusoside\n64849-39-4\n642,73\nDulcoside A\n64432-06-0\n788,87\nStevioside\n57817-89-7\n804,88\nRebaudioside A\n58543-16-1\n967,01\nRebaudioside B\n58543-17-2\n804,88\nRebaudioside C\n63550-99-2\n951,02\nRebaudioside D\n63279-13-0\n1 129,15\nRebaudioside E\n63279-14-1\n967,01\nRebaudioside F\n438045-89-7\n936,99\nRebaudioside M\n1220616-44-3\n1 291,3\nAssay\nNot less than 95 % steviolbioside, rubusoside, dulcoside A, stevioside, rebaudiosides A, B, C, D, E, F and M on the dried basis, in any combination and ratio.\nDescription\nWhite to light yellow powder, approximately between 200 and 350 times sweeter than sucrose (at 5 % sucrose equivalency).\nIdentification\nSolubility\nFreely soluble to slightly soluble in water\npH\nBetween 4,5 and 7,0 (1 in 100 solution)\nPurity\nTotal ash\nNot more than 1 %\nLoss on drying\nNot more than 6 % (105 °C, 2h)\nResidual solvents\nNot more than 200 mg/kg methanol\nNot more than 5 000 mg/kg ethanol\nArsenic\nNot more than 1 mg/kg\nLead\nNot more than 1 mg/kg\n’",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2016/1814/annex",
    "retrieved_at": "2026-11-01T14:45:28.255720+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}