# Commission Delegated Regulation (EU) 2019/2035 of 28 June 2019 supplementing Regulation (EU) 2016/429 of the European Parliament and of the Council as regards rules for establishments keeping terrestrial animals and hatcheries, and the traceability of certain kept terrestrial animals and hatching eggs (Text with EEA relevance), Division 3: Diseases surveillance for Mycoplasma spp. of relevance for poultry:

Extent: E+W+S+N.I.

3.1. Identification of infection with the following agents:
(a) Mycoplasma gallisepticum;
(b) Mycoplasma meleagridis.
3.2. Target species:
(a) Mycoplasma gallisepticum: Gallus gallus, Meleagris gallopavo;
(b) Mycoplasma meleagridis: Meleagris gallopavo.
3.3. Examinations:
Each flock must be clinically examined during each laying or productive period at the best time for detecting the disease in question.
3.4. Sampling matrix:
Samples to be taken from each flock in the establishment keeping poultry, as appropriate:
(a) blood;
(b) sperm;
(c) swabs taken from the trachea, the choanae or the cloaca;
(d) post mortem tissues, especially air sacs from day-old chicks with lesions;
(e) in particular for the detection of Mycoplasma meleagridis, oviduct and penis of turkeys.
3.5. Sampling frame and frequency of sampling:
(a) sampling for Mycoplasma gallisepticum:
Species
Time of sampling
Number of birds to be sampled
Breeding poultry
Productive poultry
Gallus gallus
at 16 weeks of age
at the point of lay
and then every 90 days
During production every 90 days
60
60
60
Meleagris gallopavo
at 20 weeks of age
at the point of lay
and then every 90 days
During production every 90 days
60
60
60
(b) sampling for Mycoplasma meleagridis:
Species
Time of sampling
Number of birds to be sampled
Breeding poultry
Productive poultry
Meleagris gallopavo
at 20 weeks of age
at the point of lay
and then every 90 days
During production every 90 days
60
60
60
(c) the number of birds to be sampled according to points (a) and (b) may be adapted by the competent authority to the known prevalence of infection in the specific Member State concerned and its past incidence in the establishment. In any case a statistically valid numbers of samples for serological/bacteriological testing shall always be taken.
3.6. Examinations, sampling and testing methods:
Testing for the presence of infection by serological, bacteriological and molecular tests must be carried out by validated methods recognised by the competent authority.
3.7. Results:
A flock is considered positive when, following the positive results of the testing performed in accordance with points 3.3 to 3.6, a second test of an appropriate type confirms the infection by the disease agents.

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Source: https://www.legislation.gov.uk/eur/2019/2035/annex/II/part/2/division/3 (the official version)
Page: https://openlegislation.uk/eu-regulation-2019-2035/annex-II/part-2/division-3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.