Annex IV REFERENCE TESTING METHOD FOR ANALYSIS OF E. COLI IN LIVE BIVALVE MOLLUSCS FOR CLASSIFICATION OF PRODUCTION AND RELAYING AREAS IN ACCORDANCE WITH ARTICLE 52(2)

The reference method for analysis of E. coli in live bivalve molluscs shall be the detection and ‘most probable number’ (MPN) technique specified in ISO 16649-3. Alternative methods may be used if they are validated against this reference method in accordance with the criteria in ISO 16140.