# Commission Implementing Regulation (EU) 2019/627 of 15 March 2019 laying down uniform practical arrangements for the performance of official controls on products of animal origin intended for human consumption in accordance with Regulation (EU) 2017/625 of the European Parliament and of the Council and amending Commission Regulation (EC) No 2074/2005 as regards official controls (Text with EEA relevance), Division 7: Calculation of TVB-N concentration

Extent: E+W+S+N.I.

By titration of the receiver solution with hydrochloric acid standard solution, the TVB-N concentration is calculated using the following equation:
TVB-N expressed in mg∕100 g sample=V1−V0×0,14×2×100Mwhere:
V1volume of 0,01 mol hydrochloric acid standard solution in ml for sample;
V0volume of 0,01 mol hydrochloric acid standard solution in ml for blank;
Mmass of sample in g.
In addition, the following is required:
(a)duplicate analyses. The applied method is correct if the difference between duplicates is not greater than 2 mg/100 g;
(b)equipment check. The equipment is checked by distilling solutions of NH4Cl equivalent to 50 mg TVB-N/100 g;
(c)standard deviations. The standard deviation for repeatability Sr = 1,20 mg/100 g and the standard deviation for reproducibility SR = 2,50 mg/100 g are calculated.

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Source: https://www.legislation.gov.uk/eur/2019/627/annex/VI/chapter/II/division/C/division/7 (the official version)
Page: https://openlegislation.uk/eu-regulation-2019-627/annex-VI/chapter-II/division-C/division-7
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.