Division 2 All bovine animals kept at a semen collection centre shall be subjected at least once a year to the following tests (compulsory routine tests), with negative results:

for infection with Mycobacterium tuberculosis complex (M. bovis, M. caprae and M. tuberculosis), an intradermal tuberculin test referred to in point 1 of Part 2 of Annex I to Delegated Regulation (EU) 2020/688;

for infection with Brucella abortus, Brucella melitensis and Brucella suis, a serological test referred to in point 1 of Part 1 of Annex I to Delegated Regulation (EU) 2020/688;

for enzootic bovine leukosis, a serological test referred to in point (a) of Part 4 of Annex I to Delegated Regulation (EU) 2020/688;

for infectious bovine rhinotracheitis/infectious pustular vulvovaginitis, a serological test (whole virus) on a blood sample;

for bovine viral diarrhoea, a serological test for the detection of an antibody which is applied only to seronegative animals.

In the event that an animal becomes serologically positive, every ejaculate of that animal collected since the last negative test shall be either discarded or tested for virus or virus genome with negative results;

for bovine genital campylobacteriosis, a test on a sample of preputial specimen. Only bulls in semen production or having contact with bulls in semen production shall be required to be tested. Bulls returning to collection after a lay-off period of more than 6 months shall be tested during a period of 30 days prior to resuming production;

for trichomonosis, a test on a sample of preputial specimen. Only bulls in semen production or having contact with bulls in semen production shall be required to be tested. Bulls returning to collection after a lay-off period of more than 6 months shall be tested during a period of 30 days prior to resuming production.