{
  "url": "https://openlegislation.uk/eu-regulation-2020-689/annex-III/section-5",
  "json_url": "https://openlegislation.uk/eu-regulation-2020-689/annex-III/section-5.json",
  "legislation": "eur/2020/689",
  "legislation_title": "Commission Delegated Regulation (EU) 2020/689 of 17 December 2019 supplementing Regulation (EU) 2016/429 of the European Parliament and of the Council as regards rules for surveillance, eradication programmes, and disease-free status for certain listed and emerging diseases (Text with EEA relevance)",
  "legislation_url": "https://openlegislation.uk/eu-regulation-2020-689",
  "path": "annex/III/section/5",
  "kind": "section",
  "number": "5",
  "heading": "Infection with Aujeszky’s disease virus (ADV)",
  "text": "ELISA for the detection of antibodies against whole ADV, ADV-gB protein or ADV-gD protein. For batch control of ADV-gB kits and ADV-gD kits or whole ADV kits, Community reference serum ADV 1, or sub-standards, must be scored positive at the dilution of 1:2. When referred to tests for the detection of whole ADV in Part V of Annex IV either of these tests may be used.\nELISA for the detection of antibodies against ADV-gE protein. For batch control, Community reference serum ADV 1, or sub-standards, must be scored positive at the dilution 1:8.\nMethods:\nMatrix:\nnon-vaccinated porcine animals\nADV ELISA\nindividual or up to 5 pooled serum (or plasma) samples\nindividual or up to 5 pooled filter paper samples\nindividual meat juice samples\nDIVA vaccinated porcine animals with a gE-deleted vaccine\ngE ELISA\nindividual serum samples",
  "extent": "E+W+S+N.I.",
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "footnote",
      "label": "f1",
      "text": "ELISA for the detection of antibodies against whole ADV, ADV-gB protein or ADV-gD protein. For batch control of ADV-gB kits and ADV-gD kits or whole ADV kits, Community reference serum ADV 1, or sub-standards, must be scored positive at the dilution of 1:2. When referred to tests for the detection of whole ADV in Part V of Annex IV either of these tests may be used."
    },
    {
      "type": "footnote",
      "label": "f2",
      "text": "ELISA for the detection of antibodies against ADV-gE protein. For batch control, Community reference serum ADV 1, or sub-standards, must be scored positive at the dilution 1:8."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/eur/2020/689/annex/III/section/5",
    "retrieved_at": "2026-11-01T14:42:38.010272+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}