# The Feeding Stuffs Regulations 2000, Schedule 8: CONTROL OF CERTAIN PROTEIN SOURCES Column 1 Column 2 Column 3 Column 4 Column 5 Column 6 Column 7 Name of product group Permitted products Designation of nutritive principle or identity of micro-organisms Culture substrate (specifications, if any) Composition characteristics of product Animal species Name of product and specified particulars (1) In this Schedule the contents laid down or to be declared in accordance with Columns 5 and 7 refer to the product as such. 1 Proteins obtained from the following groups of micro-organisms 1.1 Bacteria 1.1.1 Bacteria cultivated on methanol 1.1.1.1 Protein product of fermentation obtained by culture of Methylophilus methylotrophus on methanol Methylophilus methylotrophus NCIB strain 10.515 Methanol protein: min 68%—Reflectance index: at least 50 Pigs, calves, poultry and fish Declarations to be made on the label or packaging of the product: name of the product; protein; ash; fat; moisture content; instructions for use; “avoid inhalation”; As from 1st April 2001: approval number; Declarations to be made on the label or packaging of compound feeding stuffs: amount of the product contained in the feeding stuff 1.1.2 Bacteria cultivated on natural gas 1.1.2.1 Protein product of fermentation from natural gas obtained by culture of: Methylococcus capsulatus (Bath), Alcaligenes acidovorans, Bacillus brevis et Bacillus firmus, and the cells of which have been killed Methyloccus capsulatus (Bath) NCIMB strain 11132 Alcaligenes acidovorans NCIMB strain 12387 Bacillus brevis NCIMB strain 13288 Bacillus firmus NCIMB strain 13280 Natural gas: (approx 91% methane, 5% ethane, 2% propane, 0.5% isobutane, 0.5% n-butane, 1% other components), ammonia, mineral salts protein: min. 65% — Pigs for fattening from 25 to 60kg — Calves from 80kg on — Salmon Declarations to be made on the label or the packaging of the product: the name “Protein product of fermentation from natural gas obtained by culture of Methylococcus capsulatus (Bath), Alcaligenes acidovorans, Bacillus brevis and Bacillus firmus” protein ash fat moisture content instructions for use maximum incorporation rate in the feed: 8% pigs for fattening 8% calves 19% salmon (freshwater) 33% salmon (seawater) “avoid inhalation””; Declarations to be made on the label or packaging of the compound feedingstuffs. The name “Protein product obtained by bacterial fermentation of natural gas” amount of the product contained in the feedingstuff As from 1st April 2001: approval number 1.2 Yeasts 1.2.1 Yeasts cultivated on substrates of animal or vegetable origin — Yeasts obtained from the micro-organisms and substrates listed in columns 3 and 4, the cells of which have been killed Saccharomyces cerevisiae Saccharomyces carlsbergiensis Kluyveromyces lactis Kluyveromyces fragilis Molasses, distillery residues, cereals and products containing starch, fruit juice, whey, lactic acid, hydrolized vegetable fibres — All animal species — 1.2.2 Yeasts cultivated on substrates other than those given in 1.2.1. 1.3 Algae 1.4 Lower fungi 1.4.1 Products from production of antibiotics by fermentation 1.4.1.1 Mycelium, wet by-product from the production of penicillin, ensiled by means of lactobacillus brevis, plantarum, sake, collenoid and streptococcus lactis to inactivate the penicillin, and heat treated Nitrogenous compound Penicillium chrysogenum ATCC 48271 Different sources of carbohydrates and their hydrolysates Nitrogen expressed as protein: min. 7% Ruminants Pigs Declaration to be made on the label or packaging of the product: the name: “Mycelium silage from the production of penicillin”; Nitrogen expressed as protein; ash; moisture; animal species or category; As from 1st April 2001: approval number; Declaration to be made on the label or packaging of the compound feeding stuff: the name “mycellium silage from the production of penicillin”: 2 Non-protein nitrogenous compounds 2.1 Urea and its derivatives 2.1.1 Urea, technically pure 2.1.2 Biuret, technically pure 2.1.3 Urea-phosphate, technically pure 2.1.4 Diureidoisobutane technically pure CO(NH2)2 (CONH2)2-NH CO(NH2)2 H3 PO4 (CH3) - (CH)2 (NHCONH2)2 — — — — Urea min. 97% Biuret: min. 97% Nitrogen: min. 16.5% Phosphorus: min. 18% Nitrogen: min. 30% Isobutyraldehyde: min. 35% Ruminants from the beginning of rumination Declarations to be made on the label or packaging of the product: the name: “Urea”, “Biuret”, “Urea-phosphate” or “Diureidoisobutane”, as the case may be; nitrogen level; and in addition for product 2.1.3., phosphorus level; animal species or category; Declarations to be made on the label or packaging of compound feeding stuffs: the name “Urea”. “Biuret”, “Urea-phosphate” or “Diureidoisobutane”, as the case may be; amount of the product contained in the feeding stuff; percentage of the total protein provided by non-protein nitrogen; indication, in the instructions for use, of the level of total non-protein nitrogen which should not be exceeded in the daily ration of each animal species or category 2.2 Ammonium salts 2.2.1 Ammonium lactate, produced by fermentation with Lactobacillus bulgaricus CH3CHOHCOONH4 Whey Nitrogen expressed as protein: min. 44% Ruminants from the beginning of rumination Declarations to be made on the label or packaging of the product: the name: “Ammonium lactate from fermentation”; nitrogen expressed as protein; ash; moisture; animal species or category; Declarations to be made on the label or packaging of compound feeding stuffs: the name “Ammonium lactate from fermentation”; amount of product contained in the feeding stuff; percentage of the total protein provided by non-protein nitrogen; indication, in the instructions for use, of the level of total non-protein nitrogen which should not be exceeded in the daily ration of each animal species or category 2.2.2 Ammonium accetate in aqueous solution CH3COONH4 — Ammonium acetate: min. 55% Ruminants from the start of rumination Declarations to be made on the label or packaging of the product: the words “Ammonium acetate”; nitrogen content moisture content; animal species or category; Declarations to be made on the label or packaging of compound feeding stuffs: the words “Ammonium acetate”; the amount of the product contained in the feeding stuff; percentage of the total protein provided by non-protein nitrogen; indication in the instructions for use of the level of total non-protein nitrogen which should not be exceeded in the daily ration for each animal species or category 2.2.3 Ammonium sulphate in aqueous solution (NH4)2SO4 — Ammonium sulphate: min. 35 % Ruminants, from the start of rumination Declarations to be made on the label or packaging of the product: the words “Ammonium sulphate”; nitrogen and moisture contents; animal species; in the case of young ruminants, the incorporation rate in the daily ration may not exceed 0.5%; Declaration to be made on the label or packaging of compound feeding stuffs: the words “Ammonium sulphate”; the amount of the product contained in the feeding stuff; percentage of the total protein provided by non-protein nitrogen; indication in the instructions for use of the level of total non-protein nitrogen which should not be exceeded in the daily ration of each animal species; in the case of young ruminants, the incorporation rate in the daily ration may not exceed 0.5% 2.3 By-products from the production of amino acids by fermenation 2.3.1 Concentrated liquid by-products from the production of L-glutamic acid by fermentation with Corynebacterium melassecola Ammonium salts and other nitrogenous compounds Sucrose, molasses, starch products and their hydrolysates Nitrogen expressed as protein: min. 48% Moisture max. 28% Ruminants from the beginning of rumination Declarations to be made on the label or packaging of the product: the name “by products from the production of L-glutamic acid” in the case of product 2.3.1.; “by products- from the production of L-lysine” in the case of product 2.3.2.; 2.3.2 Concentrated liquid by-products from the production of L-lysine monohydro-chloride by fermentation with Brevibacterium lactofermentum Ammonium salts and other Nitrogenous compounds Sucrose, molasses, starch products and their hydrolysates Nitrogen expressed as protein: min. 45% Ruminants from the beginning of rumination nitrogen, expressed as protein; ash; moisture; animal species or category; As from 1st April 2001: approval number; Declarations to be made on the label or packaging of compound feeding stuffs: percentage of the total protein provided by non-protein nitrogen; indication, in the instructions for use, of the level of total non-protein nitrogen which should not be exceeded in the daily ration of each animal species or category 3 Amino acids and their salts All animal species 3.1 Methionine 3.1.1 DL-methionine, technically pure CH3S(CH2)2-CH(NH2)-COOH — DL-methionine: min. 98% Ruminants from the beginning of rumination Declarations to be made on the label or packaging of the product: the name: “DL-methionine”, in the case of product 3.1.1. “Dihydrated calcium salt of N-hydroxymethyl-DL-methionine” in the case of product 3.1.2., “Zinc-methionine”, in the case of product 3.1.3., DL-methionine and moisture content; animal species or category in the case of products 3.1.2., and 3.1.3.; 3.1.2 Dihydrated calcium salt of N-hydroxymethyl-DL-methionine, technically pure [CH3 S(CH2)2-CH(NH-CH2OH)-COO]2CA.2H2O — DL-methionine: min. 67% Formaldehyde: max. 14% Calcium: min. 9% Ruminants from the beginning of rumination Declarations to be made on the label or packaging of the product: the name: “DL-methionine”, in the case of product 3.1.1. “Dihydrated calcium salt of N-hydroxymethyl-DL-methionine” in the case of product 3.1.2., “Zinc-methionine”, in the case of product 3.1.3., DL-methionine and moisture content; animal species or category in the case of products 3.1.2., and 3.1.3.; 3.1.3 Methionine-zinc, technically pure [CH3S(CH2)2-CH(NH2)-COO]2Zn — DL-methionine: min. 80% Zn; max. 18.5% — As from 1st April 2001: approval number; 3.1.4 Concentrated liquid sodium DL-methionine technically pure (CH3S(CH2)2-CH (NH2)-COO)Na — DL-methionine: min. 40% Sodium: min. 6.2% All animal species Declarations to be made on the label or packaging of the product: the name: “concentrated liquid sodium DL-methionine”; DL-methionine content; moisture content; As from 1stApril 2001: approval number; 3.1.5 DL-methionine, technically pure protected with copolymer vinylpyridine/styrene CH3S(CH2)2-CH(NH2)-COOH — DL-methionine: minimum 65% copolymer vinylpyridine/styrene: maximum 3% Dairy cows Declarations to be made on the label or packaging of the product: “Protected methionine with copolymer vinylpyridine/styrene”; DL-methionine and moisture contents; animal species; As from 1stApril 2001: approval number; 3.2 Lysine 3.2.1 L-Lysine, technically pure 3.2.2 Concentrated liquid L-Lysine (base) 3.2.3 L-Lysine-monohydrochloride, technically pure 3.2.4 Concentrated liquid L-Lysine-monohydrochloride NH2-(CH2)4-CH (NH2)-COOH NH2-(CH2)4-CH(NH2)-COOH NH2-(CH2)4-CH(NH2)-COOH.HCl NH2-(CH2)4-CH(NH2)-COOH.HC1 — Saccharose, molasses, starch products and their hydrolysates — Saccharose, molasses, starch products and their hydrolysates L-Lysine: min. 98% L-Lysine: min. 50% L-Lysine: min. 78% L-Lysine: min. 22.4% All animal species Declarations to be made on the label or packaging of the product: the name “L-Lysine” in the case of product 3.2.1., “Concentrated liquid L-Lysine base” in the case of product 3.2.2., “L-Lysine monohydrochloride” in the case of product 3.2.3., “Concentrated liquid L-Lysine monohydrochloride” in the case of product 3.2.4., “L-Lysine sulphate and its by-products from fermentation” in the case of product 3.2.5.; 3.2.5 L-Lysine, sulphate produced by fermentation with Corynebacterium glutamicum [NH2-(CH2)4-CH (NH2)-COOH]2-H2SO4 Sugar syrup, molasses, cereals, starch products and their hydrolysates L-Lysine: min. 40% — L-Lysine and moisture content; — As from 1st April 2001: approval number; 3.2.6 L-Lysine phosphate and its by-products produced by fermentation with Brevibacterium lactofermentatum NRRLB-11470 [NH2(CH2)4-CH(NH2)-COOH]-H3PO4 Sucrose ammonia and fish solubles L-Lysine: min. 35% Phosphorus: min. 4.3% Poultry Pigs Declarations to be made on the label or packaging of the product: the name: “L-Lysine phosphate and its by-products from fermentation” L-Lysine and moisture content; As from 1st April 2001: approval number; 3.2.7 Mixtures of: (a) L-Lysine-mono-hydrochloride, technically pure and (b) DL-methionine technically pure protected with copolymer vinyl-pyridine/styrene NH2(CH2)4-CH(NH2)-COOH-HCl CH3S(CH2)2-CH(NH2)-COOH — L-Lysine + DL-methionine: minimum 50% (including DL-methionine: minimum 15%) Copolymer vinyl pyridine/styrene: maximum 3% Dairy cows Declarations to be made on the label or packaging of the product: the name: “mixture of L-Lysine monohydrochloride and DL-methionine protected with copolymer vinyl-pyridine/styrene”; L-Lysine, DL-methionine and moisture contents; animal species; As from 1st April 2001: approval number; 3.3 Threonine 3.3.1 L-Threonine, technically pure CH3CH(OH)-CH(NH2)-COOH — L-Threonine: min. 98% All animal species Declarations to be made on the label or packaging of the product: the name “L-Threonine”; L-Threonine and moisture content; As from 1st April 2001: approval number; 3.4 Tryptophan 3.4.1 L-Tryptophan, technically pure (C8H5NH)-CH2-CH(NH2)-COOH — L-Tryptophan: min. 98% All animal species Declarations to be made on the label or packaging of the product: the name: “L-Tryptophan”; L-Tryptophan and moisture content; As from 1st April 2001: approval number; 3.4.2 DL-Tryptophan, technically pure (C8H5NH)-CH2-CH(NH2)-COOH — DL-Tryptophan: min. 98% All animal species Declarations to be made on the label or packaging of the product: the name: “DL-Tryptophan”; DL-Tryptophan and moisture content; As from 1st April 2001: approval number; 4 Analogues of amino acids 4.1 Analogues of methionine 4.1.1 Hydroxy analogue of methionine 4.1.2 Calcium salt of hydroxy analogue of methionine CH3S(CH2)2-CH(OH)-COOH [CH3S-S-(CH2)-CH(OH)-COO]2Ca — — Total of acids: minimum 85% Monomer acid: minimum 65% Monomer acid: minimum 83% Calcium: minimum 12% All animal species Declarations to be made on the label or packaging of the product: if appropriate, the name (column 2); monomer acid and total acids contents in the case of product 4.1.1. and monomer acid content in the case of product 4.1.2. moisture content; animal species; As from 1st April 2001: approval number; Declarations to be made on the label or packaging of compound feeding stuffs: if appropriate, the name (column 2); monomer acid and total acids contents in the case of product 4.1.1. and monomer acid content in the case of product 4.1.2. amount of the product contained in the feeding stuff. --- Source: https://www.legislation.gov.uk/uksi/2000/2481/schedule/8 (the official version) Page: https://openlegislation.uk/feeding-stuffs-regulations-2000/schedule-8 Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.