{
  "url": "https://openlegislation.uk/feeding-stuffs-sampling-and-analysis-regulations-northern-ireland-1999/schedule-2/paragraph-5.1",
  "json_url": "https://openlegislation.uk/feeding-stuffs-sampling-and-analysis-regulations-northern-ireland-1999/schedule-2/paragraph-5.1.json",
  "legislation": "nisr/1999/296",
  "legislation_title": "Feeding Stuffs (Sampling and Analysis) Regulations (Northern Ireland) 1999",
  "legislation_url": "https://openlegislation.uk/feeding-stuffs-sampling-and-analysis-regulations-northern-ireland-1999",
  "path": "schedule/2/paragraph/5.1",
  "kind": "paragraph",
  "number": "5.1",
  "heading": "Procedure",
  "text": "Extraction of Uric Acid\nFrom dried poultry waste:\n5.1.1 Weigh to the nearest 0.001 g, about 0.4 g dried poultry waste and place in a 150 ml round-bottomed flask. Add 60 ml ethanolic formaldehyde solution (3.3), fit a reflux condenser onto the flask and heat on a steam bath for 1 hour. Cool and filter by suction through a sintered glass crucible (porosity 4) into a 100 ml graduated flask. Wash out the round-bottomed flask with 3 2 10 ml portions of ethanolic formaldehyde solution (3.3) passing each portion through the crucible into the graduated flask. Dilute to 100 ml with ethanolic formaldehyde solution and mix.\nFrom feeding stuffs:\n5.1.2 Weigh to the nearest 0.001 g, between 4 g and 5 g of prepared sample. Transfer to a glass percolation tube (4.2) fitted with a small paper cup to retain the feed. Remove the fat from the feed by extraction with light petroleum (3.10). Transfer quantitatively the defatted sample to a 150 ml round-bottomed flask and remove the residual solvent with a slow current of air. Continue as in 5.1.1, second sentence “ . . . Add 60 ml ethanolic formaldehyde solution (3.3) . . .”.",
  "extent": "N.I.",
  "status": "prospective",
  "version": "current",
  "annotations": [],
  "outstanding_effects": [
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      "affecting_title": "Feeding Stuffs (Sampling and Analysis) Regulations (Northern Ireland) 1999"
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      "affecting_title": "Feeding Stuffs (Sampling and Analysis) Regulations (Northern Ireland) 1999"
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      "affecting_title": "Feeding Stuffs (Sampling and Analysis) Regulations (Northern Ireland) 1999"
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      "affecting_title": "Feeding Stuffs (Sampling and Analysis) Regulations (Northern Ireland) 1999"
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  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/nisr/1999/296/schedule/2/paragraph/5.1",
    "retrieved_at": "2026-10-29T02:18:56.025950+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}