# Feeding Stuffs (Sampling and Analysis) Regulations (Northern Ireland) 1999, Paragraph 5.1: Procedure

Extent: N.I.
Status: prospective

Extraction of Uric Acid
From dried poultry waste:
5.1.1 Weigh to the nearest 0.001 g, about 0.4 g dried poultry waste and place in a 150 ml round-bottomed flask. Add 60 ml ethanolic formaldehyde solution (3.3), fit a reflux condenser onto the flask and heat on a steam bath for 1 hour. Cool and filter by suction through a sintered glass crucible (porosity 4) into a 100 ml graduated flask. Wash out the round-bottomed flask with 3 2 10 ml portions of ethanolic formaldehyde solution (3.3) passing each portion through the crucible into the graduated flask. Dilute to 100 ml with ethanolic formaldehyde solution and mix.
From feeding stuffs:
5.1.2 Weigh to the nearest 0.001 g, between 4 g and 5 g of prepared sample. Transfer to a glass percolation tube (4.2) fitted with a small paper cup to retain the feed. Remove the fat from the feed by extraction with light petroleum (3.10). Transfer quantitatively the defatted sample to a 150 ml round-bottomed flask and remove the residual solvent with a slow current of air. Continue as in 5.1.1, second sentence “ . . . Add 60 ml ethanolic formaldehyde solution (3.3) . . .”.

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Source: https://www.legislation.gov.uk/nisr/1999/296/schedule/2/paragraph/5.1 (the official version)
Page: https://openlegislation.uk/feeding-stuffs-sampling-and-analysis-regulations-northern-ireland-1999/schedule-2/paragraph-5.1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.