{
  "url": "https://openlegislation.uk/feedingstuffs-zootechnical-products-regulations-1999/schedule-5/part-II/paragraph-3",
  "json_url": "https://openlegislation.uk/feedingstuffs-zootechnical-products-regulations-1999/schedule-5/part-II/paragraph-3.json",
  "legislation": "uksi/1999/1871",
  "legislation_title": "The Feedingstuffs (Zootechnical Products) Regulations 1999",
  "legislation_url": "https://openlegislation.uk/feedingstuffs-zootechnical-products-regulations-1999",
  "path": "schedule/5/part/II/paragraph/3",
  "kind": "paragraph",
  "number": "3",
  "heading": null,
  "text": "REAGENTS\nNote: The suitability of a batch of aluminium oxide and of the other reagents should be tested before use by analysing a blank feed to which a known amount of meticlorpindol has been added.\n3.1. Aluminium oxide for column chromatography, 100 to 250 mesh, alkaline, Brockman activity 1.\n3.2. Ammonia (density 0.88g per ml).\n3.3. Anion exchange resin, AG1-X8 or Dowex 1-X8, 100 to 200 mesh—To convert Dowex resin in the chloride form to the acetate form add 1 litre of 6M hydrochloric acid to 350g of resin in a 3 litre beaker, and heat the mixture on a steam bath for 2 to 3 hours. Pour the slurry into a glass Buchner funnel, and wash the resin with water until the washings are free from chloride (about 6 litres of water are required). Transfer the resin to a 50mm diameter glass column having a coarse sintered-glass disc at the bottom end, and wash with sodium acetate solution (5g sodium acetate, anhydrous, dissolved in water and diluted to 100ml) until the column effluent gives only a cloudy solution on addition of silver nitrate solution. Return the resin to the glass Buchner funnel, and wash with water. Transfer the resin to a 3 litre beaker, add 1 litre of 40% v/v acetic acid solution (3.4) and heat on a steam bath for 3 hours or longer. Filter, and wash the resin again with water until the washings are free from chloride. Store the resin in water.\n3.4. Acetic acid solution 40% v/v.\n3.5. Methanol.\n3.6. Methanol solution 80% v/v.\n3.7. Ammoniacal methanol solution: dilute 1 volume of ammonia (3.2) with 19 volumes of methanol (3.5).\n3.8. Meticlorpindol standard solution: weigh, to the nearest 0.1mg, 125mg of meticlorpindol into a beaker, add 25ml of sodium hydroxide solution (2g sodium hydroxide dissolved in water and diluted to 100ml) to dissolve the meticlorpindol, transfer the solution to a 500ml graduated flask, and dilute to the mark with water. This solution contains 250μg per ml meticlorpindol.",
  "extent": null,
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [
    {
      "type": "amended",
      "affecting": "uksi/2000/1686",
      "affecting_title": "The Feedingstuffs (Zootechnical Products) and Medicated Feedingstuffs (Amendment) Regulations 2000"
    }
  ],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/uksi/1999/1871/schedule/5/part/II/paragraph/3",
    "retrieved_at": "2026-10-29T10:20:25.118862+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}