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Paragraph 3 PRINCIPLE AND DEFINITION

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Heat the sample in an Erlenmeyer flask by immersing the flask in a water bath at 50°C and maintain at this temperature for two hours (phase at 50°C). Then cool the flask in water bath at 20°C and maintain at this temperature for two hours (phase at 25°C). The combination of the two phases, first at 50°C then at 25°C, forms one thermal cycle.