{
  "url": "https://openlegislation.uk/milk-special-designation-regulations-1989/schedule-5/part-IV/paragraph-3",
  "json_url": "https://openlegislation.uk/milk-special-designation-regulations-1989/schedule-5/part-IV/paragraph-3.json",
  "legislation": "uksi/1989/2383",
  "legislation_title": "The Milk (Special Designation) Regulations 1989",
  "legislation_url": "https://openlegislation.uk/milk-special-designation-regulations-1989",
  "path": "schedule/5/part/IV/paragraph/3",
  "kind": "paragraph",
  "number": "3",
  "heading": "Reagents",
  "text": "(1) Whenever possible, reagents of analytical quality shall be used.\n(2) The buffer-substrate solution shall be prepared as follows:—\n(a) Butter solution: 3.5 g. of anhydrous sodium carbonate and 1.5 g. of sodium bicarbonate shall be dissolved in distilled or de-ionised water, and made up to one litre.\n(b) Substrate: Disodium p-nitrophenyl phosphate. The solid substrate shall be kept in a refrigerator.\n(b) Buffer-substrate solution: 0.15 g. of the substrate shall be placed in a 100 ml. measuring cylinder, and made up of 100 ml. with the buffer solution. The solution shall be stored in a refrigerator and protected from light. It shall give a reading of less than the standard marked 10 on the comparator disc A.P.T.W. or A.P.T.W.7 when viewed in transmitted light through a 25 mm. cell in the `all-purposes' comparator, distilled or de-ionised water being used for comparison. The solution shall not be used for more than one week.",
  "extent": null,
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/uksi/1989/2383/schedule/5/part/IV/paragraph/3",
    "retrieved_at": "2026-10-29T15:31:03.799102+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}