# The Milk (Special Designations) (Scotland) Order 1988, Paragraph 3: Culture medium


Bacterial count. The medium to be used shall be yeastrel milk agar, either compounded in the laboratory or prepared in accordance with the manufacturer’s directions from a granular desiccated medium and shall have the following composition:—
Yeastrel
3.0 grams
Peptone
5.0 grams
Agar
15.0 grams
Milk (fresh or spray-dried, skim or whole milk)
10.0 ml.
Distilled water
to 1 litre
Where the medium is compounded in the laboratory it shall be filtered through a pulp-paper filter.
The medium shall be sterilised either by autoclaving at 121degC. for 20 minutes or in a steamer for 30 minutes on three successive days.
The final reaction of the medium at room temperature shall be pH 7.2.

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Source: https://www.legislation.gov.uk/uksi/1988/2191/schedule/6/part/I/paragraph/3 (the official version)
Page: https://openlegislation.uk/milk-special-designations-scotland-order-1988/schedule-6/part-I/paragraph-3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.