{
  "url": "https://openlegislation.uk/milk-special-designations-scotland-order-1988/schedule-6/part-II/paragraph-3",
  "json_url": "https://openlegislation.uk/milk-special-designations-scotland-order-1988/schedule-6/part-II/paragraph-3.json",
  "legislation": "uksi/1988/2191",
  "legislation_title": "The Milk (Special Designations) (Scotland) Order 1988",
  "legislation_url": "https://openlegislation.uk/milk-special-designations-scotland-order-1988",
  "path": "schedule/6/part/II/paragraph/3",
  "kind": "paragraph",
  "number": "3",
  "heading": "Culture medium",
  "text": "Coliform test. The medium to be used shall be bile salt lactose broth, either compounded in the laboratory or prepared in accordance with the manufacturer’s directions from a granular desiccated medium and shall have the following composition:—\nPeptone\n20.0 grams\nBile salts\n5.0 grams\nSodium chloride (A.R.)\n5.0 grams\nLactose (A.R.)\n10.0 grams\nDistilled water\nto 1 litre\nBrom-cresol purple (1.6%)\n2.5 ml.\nThe medium shall be tubed in 5 ml. quantities in 150 x 16 mm. tubes provided with a rimless Durham tube (50 x 6.5 mm.) and sterilised either by autoclaving at 121degC. for 15 minutes or in a steamer for 30 minutes on three successive days.\nThe final reaction of the medium at room temperature shall be pH 7.2.",
  "extent": null,
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/uksi/1988/2191/schedule/6/part/II/paragraph/3",
    "retrieved_at": "2026-10-29T15:52:43.168909+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}