# The Milk (Special Designations) (Scotland) Order 1988, Paragraph 3: Culture medium


Coliform test. The medium to be used shall be bile salt lactose broth, either compounded in the laboratory or prepared in accordance with the manufacturer’s directions from a granular desiccated medium and shall have the following composition:—
Peptone
20.0 grams
Bile salts
5.0 grams
Sodium chloride (A.R.)
5.0 grams
Lactose (A.R.)
10.0 grams
Distilled water
to 1 litre
Brom-cresol purple (1.6%)
2.5 ml.
The medium shall be tubed in 5 ml. quantities in 150 x 16 mm. tubes provided with a rimless Durham tube (50 x 6.5 mm.) and sterilised either by autoclaving at 121degC. for 15 minutes or in a steamer for 30 minutes on three successive days.
The final reaction of the medium at room temperature shall be pH 7.2.

---
Source: https://www.legislation.gov.uk/uksi/1988/2191/schedule/6/part/II/paragraph/3 (the official version)
Page: https://openlegislation.uk/milk-special-designations-scotland-order-1988/schedule-6/part-II/paragraph-3
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.