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Paragraph 4 Dilutions

Original text. Later amendments are not shown.

Quarter-strength Ringer’s solution shall be used. The composition of full-strength Ringer’s solution shall be —

Sodium chloride9.00 grams
Potassium chloride0.42 grams
Anhydrous calcium chloride0.24 grams
Sodium bicarbonate0.20 grams
Distilled water1,000 ml.

Add 1 part of the above solution to 3 parts of distilled water. Fill into test tubes or bottles and sterilise by autoclaving at 121degC. for 15 minutes. The quantity to be filled into the container before sterilisation must be predetermined to allow for evaporation losses during sterilisation.

Alternatively, sterile tubes and bottles may be filled aseptically with measured quantities of sterile quarter-strength Ringer’s solution.