Paragraph 3 Reagents
The following reagents shall be used–
(a)
cyclohexane (analytical reagent grade);
(b)
dicholoromethane (analytical reagent grade);
(c)
hydrochloric acid 25 per centum (weight/volume);
(d)
silica gel GF 254 (Merck or equivalent);
(e)
0.5 per centum (weight/volume) solution of 2,4,7-trinitrofluorenone (TNF) (Fluka, BDH or equivalent) in acetone;
(f)
0.1 per centum (weight/volume) solution of 2,6-dibromo—p-benzoquinone-chlorimine in ethanol (stable for up to one week if kept in the refrigerator);
(g)
concentrated solution of ammonia, specific gravity: 0.9;
(h)
standard 1 per centum (weight/volume) solution of pure biphenyl in cyclohexane;
(j)
standard 1 per centum (weight/volume) solution of pure 2-hydroxybiphenyl in cyclohexane.