{
  "url": "https://openlegislation.uk/private-water-supplies-scotland-regulations-2006/schedule-5/paragraph-wrapper13n1",
  "json_url": "https://openlegislation.uk/private-water-supplies-scotland-regulations-2006/schedule-5/paragraph-wrapper13n1.json",
  "legislation": "ssi/2006/209",
  "legislation_title": "The Private Water Supplies (Scotland) Regulations 2006",
  "legislation_url": "https://openlegislation.uk/private-water-supplies-scotland-regulations-2006",
  "path": "schedule/5/paragraph/wrapper13n1",
  "kind": "paragraph",
  "number": null,
  "heading": null,
  "text": "Table A\nParameters for which, subject to regulation 31(4), methods of analysis are prescribed\n(1)Parameter\n(2)Method\nClostridium perfringens (including spores)\nMembrane filtration followed by anaerobic incubation of the membrane on m–CP agar(i) at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds.\nColiform bacteria\nISO 9308–1\nColony count 22°C – enumeration of culturable micro-organisms\nprEN ISO 6222\nEnterococci\nISO 7899–2\nEscherichia coli (E.coli)\nISO 9308–1\n(i) The composition of m–CP agar is:\nBasal medium\nTryptose\n30g\nYeast extract\n20g\nSucrose\n5g\nL–cysteine\n1g\nMgSO4 . 7H2O\n0.1g\nBromocresol purple\n40mg\nAgar\n15g\nWater\n1,000ml\nDissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool and add:\nD–cycloserine\n400mg\nPolymyxine–B–sulphate\n25mg\nIndoxyl–β-D-glucoside\nto be dissolved in 8ml sterile water before addition\n60mg\nFilter – sterilised 0.5% phenolphthalein disphosphate solution\n20ml\nFilter – sterilised 4.5% FeC13 . 6H2O\n2ml",
  "extent": null,
  "status": null,
  "version": "current",
  "annotations": [],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/ssi/2006/209/schedule/5/paragraph/wrapper13n1",
    "retrieved_at": "2026-10-28T22:20:04.570147+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}