# The Private Water Supplies (Scotland) Regulations 2006, Paragraph


Table A
Parameters for which, subject to regulation 31(4), methods of analysis are prescribed
(1)Parameter
(2)Method
Clostridium perfringens (including spores)
Membrane filtration followed by anaerobic incubation of the membrane on m–CP agar(i) at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds.
Coliform bacteria
ISO 9308–1
Colony count 22°C – enumeration of culturable micro-organisms
prEN ISO 6222
Enterococci
ISO 7899–2
Escherichia coli (E.coli)
ISO 9308–1
(i) The composition of m–CP agar is:
Basal medium
Tryptose
30g
Yeast extract
20g
Sucrose
5g
L–cysteine
1g
MgSO4 . 7H2O
0.1g
Bromocresol purple
40mg
Agar
15g
Water
1,000ml
Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool and add:
D–cycloserine
400mg
Polymyxine–B–sulphate
25mg
Indoxyl–β-D-glucoside
to be dissolved in 8ml sterile water before addition
60mg
Filter – sterilised 0.5% phenolphthalein disphosphate solution
20ml
Filter – sterilised 4.5% FeC13 . 6H2O
2ml

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Source: https://www.legislation.gov.uk/ssi/2006/209/schedule/5/paragraph/wrapper13n1 (the official version)
Page: https://openlegislation.uk/private-water-supplies-scotland-regulations-2006/schedule-5/paragraph-wrapper13n1
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.