{
  "url": "https://openlegislation.uk/water-supply-water-quality-regulations-2000/schedule-4",
  "json_url": "https://openlegislation.uk/water-supply-water-quality-regulations-2000/schedule-4.json",
  "legislation": "uksi/2000/3184",
  "legislation_title": "The Water Supply (Water Quality) Regulations 2000",
  "legislation_url": "https://openlegislation.uk/water-supply-water-quality-regulations-2000",
  "path": "schedule/4",
  "kind": "schedule",
  "number": "4",
  "heading": "ANALYTICAL METHODOLOGY",
  "text": "TABLE A1\nPARAMETERS FOR WHICH, SUBJECT TO REGULATION 16(7),METHODS OF ANALYSIS ARE PRESCRIBED\n(1)\n(2)\nParameter\nMethod\n*The composition of m-CP agar is:\nBasal medium\nTryptose\n30.0g\nYeast extract\n20.0g\nSucrose\n5.0g\nL-cysteine hydrochloride\n1.0g\nMgSO4.7H2O\n0.1g\nBromocresol purple\n40.0mg\nAgar\n15.0g\nWater\n1,000.0ml\nDissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool and add:\nD-cycloserine\n400.0mg\nPolymyxine-B sulphate\n25.0mg\nIndoxyl-β-D-glucoside\n60.0mg\nto be dissolved in 8ml sterile water before addition\nFilter-sterilised 0.5% phenolphthalein diphosphate solution\n20.0ml\nFilter-sterilised 4.5% FeCl3.6H2O\n2.0ml\nClostridium perfringens (including spores)\nMembrane filtration followed by anaerobic incubation of the membrane on m-CP agar at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds.\nColiform bacteria\nISO 9308-1\nColony count 22°C-enumeration of culturable microorganisms\nPrEN ISO 6222\nColony count 37°C-enumeration of culturable microoganisms\nprEN ISO 6222\nEnterococci\nISO 7899-2\nEscherichia coli (E. coli)\nISO 9308-1\nTABLE A2\nPARAMETERS IN RELATION TO WHICH METHODS OF ANALYSIS MUSTSATISFY PRESCRIBED CHARACTERISTICS\n(1)\n(2)\n(3)\n(4)\nParameters\nTrueness % of prescribed concentration or value or specification\nPrecision % of prescribed concentration or value or specification\nLimit of detection % of prescribed concentration or value or specification\nNotes: (i)The method of analysis should determine total cyanide in all forms.\n(ii)The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned.\n(iii)The performance characteristics apply to the individual substances specified at 25% of the parametric value in Part I of Table B in Schedule 1.\n(iv)The performance characteristics apply to the individual substances specified at 50% of the parametric value in Part I of Table B in Schedule 1.\n(v)The performance characteristics apply to the prescribed value of 4 NTU.\n(vi)The performance characteristics apply to the specification of 1 NTU for water leaving treatment works.\nAluminium\n10\n10\n10\nAmmonium\n10\n10\n10\nAntimony\n25\n25\n25\nArsenic\n10\n10\n10\nBenzene\n25\n25\n25\nBenzo(a)pyrene\n25\n25\n25\nBoron\n10\n10\n10\nBromate\n25\n25\n25\nCadmium\n10\n10\n10\nChloride\n10\n10\n10\nChromium\n10\n10\n10\nColour\n10\n10\n10\nConductivity\n10\n10\n10\nCopper\n10\n10\n10\nCyanide\n10\n10\n10\n1,2-dichloroethane\n25\n25\n10\nFluoride\n10\n10\n10\nIron\n10\n10\n10\nLead\n10\n10\n10\nManganese\n10\n10\n10\nMercury\n20\n10\n20\nNickel\n10\n10\n10\nNitrate\n10\n10\n10\nNitrite\n10\n10\n10\nPesticides and related products\n25\n25\n25\nPolycyclic aromatic hydrocarbons\n25\n25\n25\nSelenium\n10\n10\n10\nSodium\n10\n10\n10\nSulphate\n10\n10\n10\nTetrachloroethene\n25\n25\n10\nTetrachloromethane\n20\n20\n20\nTrichloroethene\n25\n25\n10\nTrihalomethanes:\n25\n25\n10\nTotal\nTurbidity\n10\n10\n10\nTurbidity\n25\n25\n25",
  "extent": null,
  "status": null,
  "version": "current",
  "annotations": [
    {
      "type": "footnote",
      "label": "f1",
      "text": "*The composition of m-CP agar is: Basal mediumTryptose30.0gYeast extract20.0gSucrose5.0gL-cysteine hydrochloride1.0gMgSO4.7H2O0.1gBromocresol purple40.0mgAgar15.0gWater1,000.0ml Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool and add: D-cycloserine400.0mgPolymyxine-B sulphate25.0mgIndoxyl-β-D-glucoside60.0mgto be dissolved in 8ml sterile water before additionFilter-sterilised 0.5% phenolphthalein diphosphate solution20.0mlFilter-sterilised 4.5% FeCl3.6H2O2.0ml"
    },
    {
      "type": "footnote",
      "label": "f2",
      "text": "(i)The method of analysis should determine total cyanide in all forms."
    },
    {
      "type": "footnote",
      "label": "f3",
      "text": "(ii)The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned."
    },
    {
      "type": "footnote",
      "label": "f4",
      "text": "(iii)The performance characteristics apply to the individual substances specified at 25% of the parametric value in Part I of Table B in Schedule 1."
    },
    {
      "type": "footnote",
      "label": "f5",
      "text": "(iv)The performance characteristics apply to the individual substances specified at 50% of the parametric value in Part I of Table B in Schedule 1."
    },
    {
      "type": "footnote",
      "label": "f6",
      "text": "(v)The performance characteristics apply to the prescribed value of 4 NTU."
    },
    {
      "type": "footnote",
      "label": "f7",
      "text": "(vi)The performance characteristics apply to the specification of 1 NTU for water leaving treatment works."
    }
  ],
  "outstanding_effects": [],
  "provenance": {
    "source": "legislation.gov.uk",
    "source_url": "https://www.legislation.gov.uk/uksi/2000/3184/schedule/4",
    "retrieved_at": "2026-10-29T08:09:45.572380+00:00"
  },
  "licence": "Open Government Licence v3.0",
  "licence_url": "https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/",
  "attribution": "Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives."
}