# The Water Supply (Water Quality) Regulations 2000, Schedule 4: ANALYTICAL METHODOLOGY


TABLE A1
PARAMETERS FOR WHICH, SUBJECT TO REGULATION 16(7),METHODS OF ANALYSIS ARE PRESCRIBED
(1)
(2)
Parameter
Method
*The composition of m-CP agar is:
Basal medium
Tryptose
30.0g
Yeast extract
20.0g
Sucrose
5.0g
L-cysteine hydrochloride
1.0g
MgSO4.7H2O
0.1g
Bromocresol purple
40.0mg
Agar
15.0g
Water
1,000.0ml
Dissolve the ingredients of the basal medium, adjust pH to 7.6 and autoclave at 121°C for 15 minutes. Allow the medium to cool and add:
D-cycloserine
400.0mg
Polymyxine-B sulphate
25.0mg
Indoxyl-β-D-glucoside
60.0mg
to be dissolved in 8ml sterile water before addition
Filter-sterilised 0.5% phenolphthalein diphosphate solution
20.0ml
Filter-sterilised 4.5% FeCl3.6H2O
2.0ml
Clostridium perfringens (including spores)
Membrane filtration followed by anaerobic incubation of the membrane on m-CP agar at 44 ± 1°C for 21 ± 3 hours. Count opaque yellow colonies that turn pink or red after exposure to ammonium hydroxide vapours for 20 to 30 seconds.
Coliform bacteria
ISO 9308-1
Colony count 22°C-enumeration of culturable microorganisms
PrEN ISO 6222
Colony count 37°C-enumeration of culturable microoganisms
prEN ISO 6222
Enterococci
ISO 7899-2
Escherichia coli (E. coli)
ISO 9308-1
TABLE A2
PARAMETERS IN RELATION TO WHICH METHODS OF ANALYSIS MUSTSATISFY PRESCRIBED CHARACTERISTICS
(1)
(2)
(3)
(4)
Parameters
Trueness % of prescribed concentration or value or specification
Precision % of prescribed concentration or value or specification
Limit of detection % of prescribed concentration or value or specification
Notes: (i)The method of analysis should determine total cyanide in all forms.
(ii)The performance characteristics apply to each individual pesticide and will depend on the pesticide concerned.
(iii)The performance characteristics apply to the individual substances specified at 25% of the parametric value in Part I of Table B in Schedule 1.
(iv)The performance characteristics apply to the individual substances specified at 50% of the parametric value in Part I of Table B in Schedule 1.
(v)The performance characteristics apply to the prescribed value of 4 NTU.
(vi)The performance characteristics apply to the specification of 1 NTU for water leaving treatment works.
Aluminium
10
10
10
Ammonium
10
10
10
Antimony
25
25
25
Arsenic
10
10
10
Benzene
25
25
25
Benzo(a)pyrene
25
25
25
Boron
10
10
10
Bromate
25
25
25
Cadmium
10
10
10
Chloride
10
10
10
Chromium
10
10
10
Colour
10
10
10
Conductivity
10
10
10
Copper
10
10
10
Cyanide
10
10
10
1,2-dichloroethane
25
25
10
Fluoride
10
10
10
Iron
10
10
10
Lead
10
10
10
Manganese
10
10
10
Mercury
20
10
20
Nickel
10
10
10
Nitrate
10
10
10
Nitrite
10
10
10
Pesticides and related products
25
25
25
Polycyclic aromatic hydrocarbons
25
25
25
Selenium
10
10
10
Sodium
10
10
10
Sulphate
10
10
10
Tetrachloroethene
25
25
10
Tetrachloromethane
20
20
20
Trichloroethene
25
25
10
Trihalomethanes:
25
25
10
Total
Turbidity
10
10
10
Turbidity
25
25
25

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Source: https://www.legislation.gov.uk/uksi/2000/3184/schedule/4 (the official version)
Page: https://openlegislation.uk/water-supply-water-quality-regulations-2000/schedule-4
Contains public sector information licensed under the Open Government Licence v3.0. Source: legislation.gov.uk, The National Archives.