Paragraph 14 I1

After the last wash each bovine embryo shall be subjected to microscopic examination at a magnification of at least 50× over its entire surface to determine that the zona pellucida is intact and is free from any adherent material. Any micromanipulation which involves penetration of the zona pellucida must be carried out in the facilities approved for the purpose, and after the last wash and examination. Such micromanipulation may only be carried out on an bovine embryo having an intact zona pellucida.