Division 2

An indirect trapping ELISA using isotype-specific monocolonal antibodies to detect swine IgG or IgM specific for swine vesicular disease virus is helpful in assessing the time of infection in the pig or on the infected premise.

In the isotype specific ELISA the viral antigen is trapped to the solid phase using an antigen-catching antibody. If the serum sample contains antibodies to swine vesicular disease virus, they are detected using an anti-pig IgG or an anti-pig IgM monoclonal antibody conjugated with peroxidase. Then, this binding is measured by means of a substrate and chromogen.

The isotype-specific ELISA may also help in distinguishing singleton reactors from true positive pigs, as referred to in (C).