Division 1

The ELISA for detection of antibodies is a monoclonal antibody-based competitive ELISA. If the serum sample contains antibodies to swine vesicular disease virus, the binding of a selected peroxidase-conjugated monoclonal antibody to virus antigen will be inhibited.

In this ELISA the swine vesicular disease viral antigen is trapped to the solid phase using monoclonal antibodies; then the sera samples are incubated at appropriate dilution, followed by the addition of the peroxidase-conjugated monoclonal antibody. Then, the inhibition of the monoclonal antibody binding is measured by means of a substrate and chromogen.