Division 2.3.5.3 Performance criteria for fluorimetric detection
United Kingdom
The performance criteria for LC methods have to be fulfilled.
This applies to molecules that exhibit native fluorescence and to molecules that exhibit fluorescence after either transformation or derivatisation. The selection of the excitation and emission wavelengths in combination with the chromatographic conditions shall be done in such a way to minimise the occurrence of interfering components in blank sample extracts.
The nearest peak maximum in the chromatogram shall be separated from the designated analyte peak by at least one full peak width at 10 % of the maximum height of the analyte peak.