Division 2
United Kingdom
The HAD technique is recommended for the identification of ASF virus isolates due to its high sensitivity and specificity. HAD is based on the capability of the ASF virus to replicate in pig macrophages and induce haemadsorption in the presence of pig erythrocytes. A characteristic "rosette" of erythrocytes develops around the infected macrophages. However, a small number of field ASF virus strains may not induce haemadsorption, but they produce a cytopathic effect. These strains may be specifically identified using the DIF test on the sediments of the cell cultures or by PCR.
Notes on Chapter VI
Textual amendments
- F1
Words in Annex Ch. 6 Section B para. 5 substituted (31.12.2020) by The Exotic Disease (Amendment etc.) (EU Exit) Regulations 2018 (S.I. 2018/1410), regs. 1, 8(7)(a)(i); 2020 c. 1, Sch. 5 para. 1(1)
- F2
Words in Annex Ch. 6 Section B para. 5 omitted (31.12.2020) by virtue of The Exotic Disease (Amendment etc.) (EU Exit) Regulations 2018 (S.I. 2018/1410), regs. 1, 8(7)(a)(ii); 2020 c. 1, Sch. 5 para. 1(1)
- F3
Words in Annex Ch. 6 Section E para. 2 omitted (31.12.2020) by virtue of The Exotic Disease (Amendment etc.) (EU Exit) Regulations 2018 (S.I. 2018/1410), regs. 1, 8(7)(b)(i); 2020 c. 1, Sch. 5 para. 1(1)
- F4
Words in Annex Ch. 6 Section E para. 2 inserted (31.12.2020) by The Exotic Disease (Amendment etc.) (EU Exit) Regulations 2018 (S.I. 2018/1410), regs. 1, 8(7)(b)(ii)(aa); 2020 c. 1, Sch. 5 para. 1(1)
- F5
Word in Annex Ch. 6 Section E para. 2 substituted (31.12.2020) by The Exotic Disease (Amendment etc.) (EU Exit) Regulations 2018 (S.I. 2018/1410), regs. 1, 8(7)(b)(ii)(bb); 2020 c. 1, Sch. 5 para. 1(1)
- F6
Words in Annex Ch. 6 Section E para. 2 omitted (31.12.2020) by virtue of The Exotic Disease (Amendment etc.) (EU Exit) Regulations 2018 (S.I. 2018/1410), regs. 1, 8(7)(b)(iii); 2020 c. 1, Sch. 5 para. 1(1)