Chapter VIII Serological tests and evaluation of results

The preferred method used to show the presence of influenza A virus is to demonstrate the possession of the nucleoprotein or matrix antigens which are shared by all influenza A viruses.

It may be done in immunodoublediffusion tests involving either concentrated virus preparations or extracts from infected chorioallantoic membranes.

The preferred methods used for serological tests for AI virus antibodies are haemagglutination (HA) and haemagglutination inhibition (Hl) tests.

Chapter 2.7.12 of the Manual of Diagnostic Tests and Vaccines for Terrestrial Animals of the World Organisation for Animal Health (OIE) contains detailed information on the laboratory techniques and the evaluation of the results.

F1The Weybridge National Reference Laboratory standard protocols for serological tests and evaluation of their results must be followed.