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Annex I Specifications of Morinda citrifolia fruit puree and concentrate

United Kingdom

Original text. Later amendments are not shown.

Description:

The fruits of Morinda citrifolia are harvested by hand. Seeds and skin are separated mechanically from the pureed fruits. After pasteurisation, the puree is packaged in aseptic containers and stored under cold conditions.

Morinda citrifolia concentrate is prepared from M. citrifolia puree by treatment with pectinolytic enzymes (50-60 °C for 1-2 h). Then the puree is heated to inactivate the pectinases and then immediately cooled. The juice is separated in a decanter centrifuge. Afterwards the juice is collected and pasteurised, prior to being concentrated in a vacuum evaporator from a brix of 6 to 8 to a brix of 49 to 51 in the final concentrate.

By an HPLC-UV method developed and validated by the applicant for the analysis of anthraquinones in Morinda citrifolia puree and concentrate.

Limits of detection: 2,5 ng/mL (5,15 dimethylmorindol); 50,0 ng/mL (lucidin); 6,3 ng/mL (alizarin) and 62,5 ng/mL (rubiadin).

Composition of Morinda citrifolia fruit puree and concentrate
Moisture89 – 93 %48 – 53 %
Protein< 0,6 g/100 g3 – 3,5 g/100 g
Fat< 0,2 g/100 g< 0,04 g/100 g
Ash< 1 g/100 g4,5 – 5 g/100 g
Total carbohydrates5 – 10 g/100 g37 – 45 g/100 g
Fructose0,5 – 2 g/100 g9 – 11 g/100 g
Glucose0,5 – 2 g/100 g9 – 11 g/100 g
Dietary fibre1,5 – 3 g/100 g1,5 – 5 g/100 g
5,15-dimethylmorindolf1 0,19 – 0,20 μg/mL0,11 – 0,77 μg/mL
Lucidin Not detectableNot detectable
Alizarin Not detectableNot detectable
Rubiadin Not detectableNot detectable

Annotations

Footnotes

f1

By an HPLC-UV method developed and validated by the applicant for the analysis of anthraquinones in Morinda citrifolia puree and concentrate.

Limits of detection: 2,5 ng/mL (5,15 dimethylmorindol); 50,0 ng/mL (lucidin); 6,3 ng/mL (alizarin) and 62,5 ng/mL (rubiadin).