Division 5.1 Methods for detection of presumptive ESBL- or AmpC- or CP-producing E. coli
United Kingdom
For the purpose of estimating the proportion of samples containing presumptive ESBL- or AmpC- or CP-producing E. coli among the caecal and fresh meat samples collected in accordance with point 1(d), the laboratories referred to in Article 3(2) shall use detection methods detailed in the protocols of the EURL for AMRf1.
All presumptive ESBL- or AmpC- or CP-producing E. coli isolates identified through the methods referred to in above shall be tested with the first panel and the second panel of antimicrobial substances in accordance with Table 2 and Table 5 respectively.
Table 5
Panel of antimicrobial substances, EUCAST epidemiological cut-off values (ECOFFs) and clinical resistance breakpoints and concentrations ranges to be used for testing only Salmonella spp. and E. coli isolates resistant to cefotaxime or ceftazidime or meropenem – (Second panel)
| NA: not available | |||||
| Antimicrobial | Class of antimicrobial | Species | Interpretative thresholds of AMR (mg/L) | Range of concentrations (mg/L)(No of wells in brackets) | |
|---|---|---|---|---|---|
| ECOFF | Clinical breakpoint | ||||
| Cefepime | Cephalosporin | Salmonella | NA | > 4 | 0,06-32 (10) |
| E. coli | > 0,125 | > 4 | |||
| Cefotaxime | Cephalosporin | Salmonella | > 0,5 | > 2 | 0,25-64 (9) |
| E. coli | > 0,25 | > 2 | |||
| Cefotaxime + clavulanic acid | Cephalosporin/beta-lactamase inhibitor combination | Salmonella | NA | NA | 0,06-64 (11) |
| E. coli | > 0,25 | NA | |||
| Cefoxitin | Cephamycin | Salmonella | > 8 | NA | 0,5-64 (8) |
| E. coli | > 8 | NA | |||
| Ceftazidime | Cephalosporin | Salmonella | > 2 | > 4 | 0,25-128 (10) |
| E. coli | > 0,5 | > 4 | |||
| Ceftazidime + clavulanic acid | Cephalosporin//beta-lactamase inhibitor combination | Salmonella | NA | NA | 0,125-128 (11) |
| E. coli | > 0,5 | NA | |||
| Ertapenem | Carbapenem | Salmonella | NA | > 0,5 | 0,015-2 (8) |
| E. coli | NA | > 0,5 | |||
| Imipenem | Carbapenem | Salmonella | > 1 | > 4 | 0,12-16 (8) |
| E. coli | > 0,5 | > 4 | |||
| Meropenem | Carbapenem | Salmonella | > 0,125 | > 8 | 0,03-16 (10) |
| E. coli | > 0,125 | > 8 | |||
| Temocillin | Penicillin | Salmonella | > NA | NA | 0,5-128 (9) |
| E. coli | > 16 | NA | |||
Annotations
Footnotes
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https://www.eurl-ar.eu/protocols.aspx