Division 2.2.2.2 F1F2F3

Standardisation of the test procedure for pooled serum samples:

1. (a)

   a 1/150 pre-dilution of the OIEISS or a 1/2 pre-dilution of the OIEELISA _WP SS or a 1/16 pre-dilution of the OIEELISA _SP SS made up in a negative serum (or in a negative pool of sera) and again diluted in negative sera by the number of samples making up the pool must give a positive reaction;

2. (b)

   the OIEELISA _N SS must always give a negative reaction;

3. (c)

   the test must be adequate to detect evidence of infection in a single animal of the group of animals, of which samples of serum have been pooled.