Division 2.2.2.1 F1F2F3

Standardisation of the test procedure for individual serum samples:

1. (a)

   a 1/150 pre-dilution ^f1 of the OIEISS or a 1/2 pre-dilution of the OIEELISA _WP SS or a 1/16 pre-dilution of the OIEELISA _SP SS made up in a negative serum (or in a negative pool of sera) must give a positive reaction;

2. (b)

   a 1/600 pre-dilution of the OIEISS or a 1/8 pre-dilution of the OIEELISA _WP SS or a 1/64 pre-dilution of the OIEELISA _SP SS made up in a negative serum (or in a negative pool of sera) must give a negative reaction;

3. (c)

   the OIEELISA _N SS must always give a negative reaction.