Division 2.3.4 F1F2F3

In carrying out the complement fixation test, the following controls must be made each time:

1. (a)

   control of the anti-complementary effect of the serum;

2. (b)

   control of the antigen;

3. (c)

   control of sensitised red blood cells;

4. (d)

   control of the complement;

5. (e)

   control using a positive serum of sensitivity at the start of the reaction;

6. (f)

   control of the specificity of the reaction using a negative serum.