Division 2.6.3 F1F2F3
United Kingdom
The antigen shall be prepared without reference to the cell concentration, but its sensitivity must be standardised in relation to the OIEISS in such a way that the antigen produces either a 50 % agglutination with a final serum dilution of 1/600 to 1/ 1 000 or 75 % agglutination with a final serum dilution of 1/500 to 1/750.
It may also be advisable to compare the reactivity of new and previously standardised batches of antigen using a panel of defined sera.